Asst. Prof. Dr. Fevziye Celebi-Toprak

Molecular Biology Division

Department of Biology

Pamukkale University

Merkez Kampus, Kinikli,

Denizli

E-mail: fctoprak@pau.edu.tr

 Phone: (0258) 2963671

Fax: (0258) 2963535

 

 

EDUCATION BACKGROUND:

Ph.D. in the Department of Plant Pathology.

Cornell University, Ithaca, NY, January 1998- May, 2001. 

MS in the Department of Plant Pathology. Cornell University, Ithaca, NY, January 1996-1998.      

MS in the Department of Biology-Biochemistry. Ankara University. October 1992-January 1994 (she didn’t complete the degree because she was awarded Turkish Ministry of Education Scholarship for MS and Ph.D in USA-February 1994) 

BS in Biology. Ankara University, Ankara, Turkey, June 1992. 
 

ACADEMIC AND PROFESSIONAL EXPERIENCE:  

Asst. Prof. in Molecular Biology Division, Pamukkale University, Turkey (July 2002-Now)
Visiting Prof. at Tsukuba University, Gene Research Center, Tsukuba, Japan (June 2002-January 2003) 
Research Asst. in Molecular Biology Division, Pamukkale University, Turkey (Jan.-July 2002)
Research Asst. in Molecular Biology Division, Adnan Menderes University, Turkey (August 2001-January 2002) 
Research Asst. in the Department of Plant Pathology, Cornell University, Ithaca, NY, USA (January 2001- May, 2001)

TEACHING EXPERIENCE

PAU: Pamukkale University, Denizli, Turkey,
ADU: Adnan Menderes University, Aydin, Turkey


PAU 2008/2009 Fall

BIO 309 Genetics I
BIO 345 Genetics Lab

BIO 408 Final Project

BIO 633 Molecular Plant Virology

PAU 2007/2008 Spring

BIO 310 Genetics II
BIO 432 Genetic Engineering
BIO 406 Molecular Gen Biology
BIO 408 Final Project  
BIO 582 Plant
Biotechnology

PAU 2007/2008 Fall

BIO 309 Genetics I
BIO 345 Genetics Lab

BIO 408 Final Project
 
BIO 572 Technique in Molecular Biology I

PAU 2006/2007 Spring

BIO 310 Genetics II
BIO 406 Molecular Gen Biology
BIO 432 Genetic Engineering
BIO 408 Final Project

PAU 2005/2006 Fall

BIO 309 Genetics I
BIO 345 Genetics Lab

BIO 408 Final Project

BIO *** DNA Repairing

PAU 2004/2005 Spring

BIO 310 Genetics II
BIO 312 Genetic Lab II
BIO 406 Molecular Gen Biology
BIO 408 Final Project

PAU 2004/2005 Fall

BIO 309 Genetics I
BIO 311 Genetics Lab I
BIO *** Recombinant DNA
BIO *** Advanced Plant Genetics

PAU 2003/2004 Spring

BIO 310 Genetics II
BIO 312 Genetic Lab II
BIO 406 Molecular Gen Biology
BIO 432 Genetic Engineering

PAU 2002/2003 Spring

Biochemistry Lab
Physiology Lab
Genetics Lab I

ADU 2001/2002 Fall

Genetics,Genetics Lab

EXPERIENCED AREAS:
Molecular Genetics, Gen Mapping, Transgenic Plants, Viruses, Quantitative RT-PCR, PVX, PVY, CMV, PLRV, PVA, In vitro Grafting, Resistance in Plants to Virus, Temperature, Drought, and Salt, Potato, Tissue culture.
 

HONORS AND AWARD:
 
Publication Incentive Awards 2007 

Publication Incentive Awards 2003 

Ranked first among 300 graduates, who took Turkish Ministry of National Education Examination in Biology, and was awarded Turkish Ministry of Education Scholarship for MS and Ph.D. 1994

Graduated as the top student in Biology at Ankara University 1992. 

Awarded Cornell University Research Assistanship- 2001 

PUBLICATIONS:  
ARTICLES
 

Fevziye ÇELEBİ-TOPRAK, Eminur BARUTÇU, Anne FRARY and Sami DOĞANLAR. Identification of Potato Y Potyvirus (PVYO) Resistance in Wild and Cultivated Tomatoes. Turkish Journal of Agriculture and Forestry (33) (2009) (Accepted available online)

Babak
 Behnam, Akira Kikuchi, Fevziye Celebi-Toprak, Mie Kasuga, Kazuko Yamaguchi-Shinozaki and Kazuo N. Watanabe  ‘Arabidopsis rd29A::DREB1A enhances freezing tolerance in transgenic potato’’ Plant Cell Reports (2007) 26, (8), 1275-1282 

Babak Behnam, Akira Kikuchi, Fevziye Celebi-Toprak, Shinsuke Yamanaka, Mie Kasuga, Kazuko Yamaguchi-Shinozaki, Kazuo N. Watanabe.  The Arabidopsis DREB1A gene driven by the stress-inducible rd29A promoter increases salt-stress tolerance in proportion to its copy number in tetrasomic tetraploid potato (Solanum tuberosum) Plant Biotechnology 23, 169–177 (2006)

Celebi-Toprak, F., B. Behnam, G. Serrano, M. Kasuga, K. Yamaguchi-Shinozaki, H. Naka, J. A. Watanabe, S. Yamanaka and K. N. Watanabe ‘Tolerance to Salt Stress of the Transgenic Tetrasomic Tetraploid Potato, Solanum tuberosum cv. Desiree Appears to be Induced by the DREB1A Gene and rd29A Promoter of Arabidopsis thaliana’ Breeding Sciences 2005 55(3):311-319

 Celebi-Toprak, F., S.A. Slack, and Russo, P., ‘’Potato Resistance to Cucumber Mosaic Virus is Temperature sensitive and Virus-train Specific’’. Breeding Science, 2003, 53: 69-74

 Celebi-Toprak , F., Slack, S. A., and Jahn, M. M.,  A New Gene Nytbr for Hypersensitivity to Potato virus Y from Solanum tuberosum Maps to Chromosome IV Theoretical Applied Genetics 2002, 104 (4) 669-674

 Celebi, F., Russo, P., Watanabe, K., Valkonen, J. P. T., and Slack, S. A. “Resistance of Potato to Cucumber Mosaic Virus Appears Related to Localization in Inoculated Leaves”.American Journal of Potato Research 1998, 75 (4), 195-199. 

 CHAPTER in BOOK  

F. Celebi-Toprak, J. A. Watanabe and K. N. Watanabe Genetic Improvement of Solanaceous Crops, Vol. 1: Potato” (Eds. M.K. Razdan and Autar K. Mattoo), “Chapter 6: Molecular Markers in Identification of Genotypic Variation” Science Publishers, USA, ISBN 1-57808-184-X  2005

 PRESENTATIONS AND CONFERENCES

Nazan Keskin, Fevziye Çelebi-Toprak, Ali Yildirim and Aysegul Demirhan Consanguineous Marriages in Saraykoy-Denizli, Turkey. The 4th International Statistics Congress May 8-12. 2005 


Behnam
, B‚ Eakhlas U. A., Celebi-Toprak, F., Kasuga M., Yamaguchi-Shinozaki K., Watanabe K. N Multiple stress tolerances induced in transgenic potatoes with rd29A and DREB1A.  The 22th Symposium, Japan Soc. Plant Cell and Molecular Biology, Akita, August 9-10, 2004  


F. Celebi-Toprak, B. Behnam, G. Serrano, H. Naka, M. Kasuga, K. Yamaguchi-Shinozaki, J. A. Watanabe, and K. N. Watanabe ‘’High Level of Tolerance to Salt Stress in the Transgenic Tetrasomic Tetraploid Potatoes’’. Breed. Res. The 105th Meeting of the Japan Society for Breeding, Univ. of Tokyo 6 Suppl (1) :272. March 30-31, 2004.

Celebi-Toprak, F. and Slack, S. A. Resistance to Cucumber Mosaic Virus in Potato Appears to Be Temperature Sensitive. Annual eeting of the American Phytopathological Society, New Orleans, USA, August 12-16, 2000. Phytopathology, 2000, Phytopathology 90:S12.

Celebi, F., and Slack, S. A. Resistance to Potato X Potexvirus and Potato Y Potyvirus in a Solanum tuberosum and Solanum berthaultii Segregating Population. Annual Meeting of the American Phytopathological Society, Montreal, Canada, August 7-11, 1999. Phytopathology, 1999, 89:S12.

Celebi, F., Russo, P., Watanabe, K., Valkonen, J. P. T., and Slack, S. A. Resistance of Potato to Cucumber Mosaic Virus Appears Related to Localization in Inoculated Leaves. Annual Meeting of the American Phytopathological Society, Rochester, New York, USA, August 9-13, Phytopathology, 1997, 87 (6 Suppl.), S16.
 

National Meeting Abstracts

Fevziye CELEBI-TOPRAK, Eminur BARUTCU Sami DOGANLAR ve Anne FRARY. 2008. Identification resistance gene to PVY in tomato 19.National Biology Kongres. 23-27 June (oral presentation)

Fevziye CELEBI-TOPRAK, Eminur BARUTCU, Anne FRARY ve Sami DOGANLAR. 2008. Identification resistance gene to PVX in potato. 19. National Biology Congress. 23-27 June (poster presentation)


National  Congress

14. Biyoteknoloji Kongresi, 31 Aðustos 2 Eylül 2005, Eskişehir, Türkiye


Seminars

Sabancı University  ‘’Identification and Mapping Virus Resistance Genes in Potato’’ 2001

Yeditepe University ‘’Identification and Mapping the New Virus Resistance Genes in Potato; and Freezing and Salt Stress Tolerance in Transgenic Potato’’ 2007

 

Projects


Assist. Prof. Dr. Fevziye ÇELEBİ-TOPRAK
Expansion of the European Turkish Business Center (BCs) Network Project, Turkey.
Windows Project
Hybrid Seed Development Project

Assist. Prof. Dr. Fevziye ÇELEBİ-TOPRAK
Building a Greenhouse, and Set up the Research Laboratory and Tissue Culture Room For Hybrid Seed Development
Pamukkale University Infrastructures Rersearch Project

CITED PUBLICATION

 

Select

Times 
Cited**

Cited Author

Cited Work [SHOW EXPANDED TITLES]

Year

Volume

Page

Article
ID

View
Record 

3

CELEBITOPRAK F

BREEDING SCI

2005

55

311

 

View Record

2

CELEBITOPRAK F

GENETIC IMPROVEMENT

2005

2

115

 

 

8

CELEBITOPRAK F

THEOR APPL GENET

2002

104

669

 

View Record

5

CELEBI F

AM J POTATO RES

1998

75

195

 

View Record

2

CELEBI F

PHYTOPATHOL S

1997

87

S16

 

View Record

3

CELEBI-TOPRAK F

PLANT CELL REPORTS   

2007

26

1275

 

 

 

 RESEARCH
Title: Identification and molecular mapping of genes conferring resistance to PVX from Solanum tuberosum population

Funding Agency: TUBITAK
Duration: June 2005 - June 2008
 Title: Identification of resistance gene(s) against to Potato Y potyvirus (PVY) in tomatoes (Lycopersicon esculentum Mill.).

Funding Agency:
Pamukkale University  
Molecular Mapping of Resistance Gene to PVX in diploid Solanum tuberosum population

Funding Agency: Pamukkale University
Duration: June 2006 - June 2009Professional Memberships: Japanese Society of Breeding (Breeding Science)

RESEARCH INTERESTS

Plant molecular genetics and. plant viruses (CMV, PVX, PVY, PLRV,PVA), resistant gene mechanism and gene mapping studies.

Transgenic plants:Arabidopsis DREB gene was used to study responses to drought, salinity, and changes in temperature in engineered crops (potatoes) to develop drought, salt and temperature resistant lines.  

Turkey is a major producer and consumer of potato and tomato. However, various diseases limit optimal production in terms of both quality and quantity (yield). One disease of both local and worldwide importance is viruses . Compared to bacterial and fungal diseases, viral pathogens are particularly difficult to combat as there is no direct way to killing viruses. Therefore, extreme care must be taken to plant only virus-free material and to prevent contamination from infected plants and farm machinery and tools. An alternative is the use of virus resistant cultivars. Although several resistant cultivars have been developed, none of the cultivars grown in Turkey carry such resistance. Recently, molecular technology has been used in plant breeding studies especially for important crop species such as potato and tomato. The development of molecular markers and molecular mapping has made it possible to transfer economically important genes. In the last few years, molecular markers have been used to facilitate the identification and transfer of virus resistance gene(s) from unadapted wild germplasm to elite cultivated types.  

My research interests primarily center around potato and tomato. My projects involve plant genetics and concern the genetic control of novel traits derived from wild species such as virus resistance in tomato and potato.  In addition to new projects in the above research areas, I and my colleagues will also identify a resistance gene against to potato X potexvirus (PVX) and molecular mapping of these gene(s) in Solanum tuberosum population. And also we identified new PVY resistant gene(s) in wild tomato Species.  

In previous work, a newly identified monogenic dominant gene, Nytbr, from S. tuberosum (USW2230) for dominant hypersensitivity to PVY° mapped to chromosome IV in a backcross population. This locus is clearly distinct from previously reported loci in potato that control the outcome of PVY infection based on both phenotype and map position. 

Plants suffer from the environmental stresses, such as drought, salinity, and cold. Because of these stresses, growth and yield of plants are dramatically reduced. In nature, when plants are exposed to the stresses, some plants develop tolerance but it takes long time. Transgenic approach using the stress responding gene such as DREBs would be promising for altering the damage given to the crops. 

I am also interested in other applications of plant tissue culture and genetic transformation for crop improvement. In previous work, we have transformed tetrasomic tetraploid potatoes with the DREB1A gene under the control of the stress inducible rd29A promoter to test whether polyploid transgenic potatoes could show relevant expression of the gene and consequently give increased tolerance to elivated levels of salinity. In addition, quantitative real-time reverse trascription PCR (TaqMan) was used to correlate DREB1A mRNA levels and apparent tolerance to salinity. Also we have correlation studies between transgene copy numbers, phenotype characterization and gene expression among stress tolerant transgenic lines.